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Via Pietro Castellino 111
80131 Naples
Tel. 0039 081 6132 579
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Amministrazione trasparente
Simone Di Paola
Current Position:
Researcher
Cell Regulation LAB
Contacts:
Phone: +39 0816132578
Lab: +39 0816132495
Email: simone.dipaola@cnr.it
s.dipaola@ieos.cnr.it
Keywords:
ADP-ribosylation; PARP; Cell Cycle; High content ScreeningMy research activity is focused on the study of mono-ADP-ribosylation (MARylation), a post-translational modification of proteins that is involved in the regulation of a wide variety of cellular functions.
Currently, I am deciphering the role of intracellular MARylating enzymes, named PARPs, in the regulation of the cell cycle, stress and organelle organization. Further, I am investigating the putative cross-talk between PARPs and enzymes able to catalyze different post-translational modifications.
The goal of my research activity is to identify the signaling pathways regulated by PARPs to potentially target them as a strategy for the treatment of human diseases. Protein biochemistry, immunofluorescence, genome editing (CRISPr/Cas9), super-resolution and electron microscopy are among the techniques used to address all the biological questions that arise from my investigations.
Moreover, I will be involved in the set-up, running and analysis of cell-based high-content
screening (HCS) assays using state-of-the-art automated microscopy technologies, with the aim of identify drugs or genes that could be novel candidates for diseases therapy.
Education
2012: PhD in Life and Biomolecular Sciences, Open University, Milton Keynes, UK and Department of Cell Biology and Oncology, Consorzio “Mario Negri Sud”, Santa Maria Imbaro, Chieti, Italy.
2006: Master degree in Medical Biotechnology (cum laude), University of Naples “Federico II”, Naples, Italy.
Positions
2020-present: Tenured Researcher; National Research Council of Italy, Naples, Italy.
2013-2020: PostDoc; Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli, Naples, Italy
2012: PostDoc; Department of Cell Biology and Oncology, Consorzio “Mario Negri Sud”, Santa Maria Imbaro, Chieti, Italy.
2007-2012: PhD student; Department of Cell Biology and Oncology, Consorzio “Mario Negri Sud”, Santa Maria Imbaro, Chieti, Italy.
Di Paola, S., Matarese, M., Barretta, M.L., Dathan, N., Colanzi, A., Corda, D., Grimaldi, G. (2022). PARP10 Mediates Mono-ADP-Ribosylation of Aurora-A Regulating G2/M Transition of the Cell Cycle. Cancers (Basel) 14, 5210.
Medina DL., Di Paola S., Peluso I., Armani A., De Stefani D., Venditti R., Montefusco S., Scotto-Rosato A., Prezioso C., Forrester A., Settembre C., Wang W., Gao Q., Xu H., Sandri M., Rizzuto R., De Matteis MA., Ballabio A. (2015). Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB. Nat Cell Biol. 2015 Mar;17(3):288-99.
Fabrizio G.*, Di Paola S.*, Stilla A, Giannotta M., Ruggiero C., Menzel S., Koch-Nolte F., Sallese M., Di Girolamo M. (2015). ARTC1-mediated ADP-ribosylation of GRP78/BiP: a new player in endoplasmic-reticulum stress responses. Cell Mol Life Sci. 2015 Mar;72(6):1209-25 (*co-first author).
Di Girolamo M., Fabrizio G., Scarpa ES and Di Paola S. (2013). NAD+-dependent enzymes at the endoplasmic reticulum. Curr. Top. Med. Chem. 2013; 13(23):3001-10. Review.
Di Paola, S., Micaroni, M., Di Tullio, G., Buccione, R., and Di Girolamo, M. (2012). PARP16/ARTD15 Is a novel endoplasmic-reticulum-associated mono-ADP-ribosyl transferase that interacts with, and modifies Karyopherin-β1. PLoS ONE 2012; 7(6):e37352.
2023-2025: Principal Investigator - PRIN (Progetti di Rilevante Interesse Nazionale) PNRR 2022 (P2022FYSY): ER-MOnoART regulaTION in proteostasis networks (EMOTION) - granted by Italian Ministry of University and Research (MUR)
2023-2025: Research Unit Coordinator - PRIN (Progetti di Rilevante Interesse Nazionale) 2022 (2022JFKF8A): MITOchondrial dynamic modulators from the SEAs (MITOSEAs) - granted by Italian Ministry of University and Research (MUR)