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Via Pietro Castellino 111
80131 Naples
Tel. 0039 081 6132 579
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Amministrazione trasparente
Mikhail A Zhukovsky
Current Position:
Researcher, TD
Contacts:
Morpho-functional organization and regulation of the biosynthetic pathway lab
Phone: +39 0816132339
Lab: +39 0816132567
Email: m.zhukovsky@ieos.cnr.it
Keywords:
Cell biology, Golgi complex, cancerUsing theoretical considerations, sequence alignments, as well as various bioinformatics softwares, I study two biological phenomena:
1) membrane fission mediated by CtBP1-S/BARS and other proteins;
2) Golgi localization of glycosylation enzymes and the role of protein adaptors, such as oncoprotein GOLPH3, in this process.
In the area of fission, I search for putative amphipathic helices of fission proteins and study the role of these helices in fission. I also investigate the interaction of fission proteins with other proteins belonging to the protein complex required for fission. I study lipid cofactors that are required for fission mediated by different proteins and search for sequence motifs that are involved in the interaction of fission proteins with these cofactors. I describe and characterize various subfamilies of fission proteins.
In the area of Golgi localization of glycosylation enzymes, I study the interaction of these enzymes with various proteins, such as COPI coatomer and adaptors, that play a role in the enzyme localization. I search for sequence motifs and binding sites that might be involved in this interaction. I propose experiments that aim to test the hypotheses concerning the putative biological role of these motifs and binding sites.
EDUCATION:
1993-1997 Kandidat nauk (PhD) in Physics, St. Petersburg State University,
1987-1993 Spezialist in Physics, Faculty of Physics, St. Petersburg State University
EMPLOYMENT:
2023-present Ricercatore III Livello (Researcher Level III)
2015-2023 Assegnista di Ricerca (Post-doctoral Fellow) IEOS-CNR [previously Institute of Protein Biochemistry (IBP)
2014-2015 Research Scientist, Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, Missouri, USA.
2008-2013 Researcher, Department of Biological Experimental Physics, Saarland University, Saarbrücken, Germany.
2007-2008 Post-doctoral Fellow, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
2002-2007 Post-doctoral Fellow, Harvard Medical School (HMS), Boston, Massachusetts,
1998-2002 Post-doctoral Fellow, National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, Maryland, USA.
1997-1998 Researcher, Department of Molecular Biophysics, Institute of Physics, St. Petersburg State University, St. Petersburg, Russia.
1995-1996 Visiting Graduate Student, Department of Biophysics, Stockholm
AWARDS AND FELLOWSHIPS:
2002-2007 Dana-Farber Cancer Institute Post-doctoral Fellowship Award
1998-2002 National Institutes of Health Post-doctoral Fellowship Award
1995-1996 Swedish Institute Scholarship for the work at the Department of Biophysics, Stockholm University
PROFESSIONAL AFFILIATIONS:
German Biophysical Society (Frankfurt am Main, Germany)
R. Rizzo, D. Russo, K. Kurokawa, P. Sahu, B. Lombardi, D. Supino, M. A. Zhukovsky, A. Vocat, P. Pothukuchi, V. Kunnathully, L. Capolupo, G. Boncompain, C. Vitagliano, F. Z. Marino, G. Aquino, D. Montariello, P. Henklein, L. Mandrich, G. Botti, H. Clausen, U. Mandel, T. Yamaji, K. Hanada, A. Budillon, F. Perez, S. Parashuraman, Y. A. Hannun, A. Nakano, D. Corda, G. D’Angelo, A. Luini, Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3, EMBO Journal (2021), 40 (8), e107238.
M. A. Zhukovsky, A. Filograna, A. Luini, D. Corda, C. Valente, Protein amphipathic helix insertion: A mechanism to induce membrane fission, Frontiers in Cell and Developmental Biology (2019), 7, 291.
M. A. Zhukovsky, A. Filograna, A. Luini, D. Corda, C. Valente, The structure and function of acylglycerophosphate acyltransferase 4/ lysophosphatidic acid acyltransferase delta (AGPAT4/LPAATδ), Frontiers in Cell and Developmental Biology (2019), 7, 147.
M. A. Zhukovsky, A. Filograna, A. Luini, D. Corda, C. Valente, Phosphatidic acid in membrane rearrangements, FEBS Letters (2019), 593, 2428-2451.
A. Pagliuso, C. Valente, L. L. Giordano, A. Filograna, G. Li, D. Circolo, G. Turacchio, V. M. Marzullo, L. Mandrich, M. A. Zhukovsky, F. Formiggini, R. S. Polishchuk, D. Corda, A. Luini, Golgi membrane fission required the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase d, Nature Communications (2016), 7, 12148.