Where we are
Via Pietro Castellino 111
80131 Naples
Tel. 0039 081 6132 579
Links
Amministrazione trasparente
Fabiola Mascanzoni
Current Position:
Researcher TD
Contacts:
The Golgi Mitotic Checkpoint lab
Phone:+39 08161325645
Email: f.mascanzoni@ieos.cnr.it
Keywords:
Golgi complex, mitosis, spindle, cell cycle, cancerMy research activity is focused on investigating the physiological role of Golgi complex disassembly during the mitotic process. In addition to the control of chromosome segregation, which is required to preserve the genetic information, the cells actively monitor the disassembly and redistribution of subcellular organelles in mitosis. The Golgi complex plays an important role in membrane traffic, but it is also actively involved in regulating mitotic entry and progression.
Our studies have demonstrated that the cells with an intact Golgi ribbon show profound alterations of fundamental mitotic processes, including the formation of multipolar spindles and binucleation, suggesting the existence of a “Golgi checkpoint”, able to integrate the stimuli between centrosome and a correct bipolar spindle formation with a consequent normal process of cell division.
Our findings open new frontiers for cancer therapy, as the identification of a novel mechanism involved in controlling spindle formation can be exploited to induce mitotic catastrophe in tumor cells by defining a combinatorial chemotherapeutic strategy able to target Golgi unlinking and other crucial mitotic functions, increasing drug potency and efficacy and enhancing antitumoral activity.
Education
- May 2016: Phd in Pharmaceutical Science, University of Naples (Federico II)
- December 2011: Master Degree in Pharmacy, University of Naples (Federico II)
Positions
- January 2023 – present: TD researcher at the Institute of Endocrinology and Experimental Oncology (IEOS), National Research Council of Italy, Naples.
- 2022-2023: Post-Doctoral fellow at the Institute of Endocrinology and Experimental Oncology (IEOS), National Research Council of Italy, Naples
- 2018-2022: Post-Doctoral fellow at the Institute of Biochemistry and Cell Biology (IBBC) National Council of Research, Naples
- 2017-2018: Post-Doctoral fellow at the the Institute of Protein Biochemistry (IBP), National Council of Research (CNR), Naples.
- 2015-2017: Fellow at Institute of Biostructure and Bioimaging, (IBB), National Research Council (CNR) Naples (PhD experience)
- 2012-2015: Fellow at the CIRPeB Interuniversity Research Centre on Bioactive Peptides, University of Naples (Federico II) in collaboration with Institute of Biostructure and Bioimaging, (IBB), National Research Council (CNR) Naples (PhD experience)
- 2010-2011: Thesis experience at Department of Organic Chemistry in Pharmacy, University of Naples, (Federico II)
- Mascanzoni F., Ayala I., Iannitti R., Luini A., Colanzi A. The Golgi checkpoint: Golgi unlinking during G2 is required for correct spindle formation and cytokinesis. Life Science Alliance ,2024. In press
- Mascanzoni F, Iannitti R, Colanzi A. Functional Coordination among the Golgi Complex, the Centrosome and the Microtubule Cytoskeleton during the Cell Cycle. Cells. 2022 Jan 21;11(3):354. doi: 10.3390/cells11030354. PMID: 35159164; PMCID: PMC8834581.
- Russo L*, Mascanzoni F*, Farina B, Dolga AM, Monti A, Caporale A, Culmsee C, Fattorusso R, Ruvo M, Doti N. Design, Optimization, and Structural Characterization of an Apoptosis-Inducing Factor Peptide Targeting Human Cyclophilin A to Inhibit Apoptosis Inducing Factor-Mediated Cell Death. J Med Chem. 2021 Aug 12;64(15):11445-11459. doi: 10.1021/acs.jmedchem.1c00777. Epub 2021 Aug 2. PMID: 34338510.
- Inizio modulo
- Mascanzoni F, Ayala I, Colanzi A. Organelle Inheritance Control of Mitotic Entry and Progression: Implications for Tissue Homeostasis and Disease. Front Cell Dev Biol. 2019 Jul 23;7:133. doi: 10.3389/fcell.2019.00133. PMID: 31396510; PMCID: PMC6664238.
- Caporale A*, Mascanzoni F*, Farina B, Sturlese M, Di Sorbo G, Fattorusso R, Ruvo M, Doti N. FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening. J Biomol Screen. 2016 Aug;21(7):701-12. doi: 10.1177/1087057116650402. Epub 2016 May 16. PMID: 27185744.