Daniela Spano

Daniela Spano
Current Position:



Golgi Mitotic Checkpoint Lab

Lab: +39 08161325645
Email: d.spano@ieos.cnr.it
Orcid ID https://orcid.org/0000-0003-3567-6492
Scopus Author ID 15048830000

Molecular Oncology, Cellular and Molecular Biology, Biochemistry, Golgi Complex, GRASP65

The main interest of Dr. Spano is the molecular oncology. Her studies are focused on the unravelling the molecular basis of cancer development by dissecting the functional role of proteins/enzymes of interest in the regulation of signaling pathways and cellular processes involved in cancer development and progression. Her field is not only the basic research but also the translational research. Indeed, she performs in vitro functional studies in order to assess the anti-cancer properties of small molecules that are able to target the proteins/enzymes object of her studies. Actually, the main subject of study is GRASP65, a Golgi “matrix” protein involved in formation of the membranous tubules connecting the stacks of Golgi complex. During G2 phase the unlinking of the Golgi ribbon into isolated stacks is regulated by JNK2-mediated phosphorylation of GRASP65. This process is required for entry into mitosis. Dr. Spano is studying the molecular mechanisms and the signaling pathways underlying the GRASP65-mediated Golgi unlinking and the entry into mitosis.


February 2003 PhD in Cellular and Molecular Biology and Pathology XIV cycle at the University of Naples “Federico II”
February 1997 Degree with full marks and honours in Biology at Faculty of Mathematical, Physical and Natural Sciences of the University of Naples “Federico II”.


11/10/2022-present: Researcher Institute Experimental Endocrinology and Oncology "Gaetano Salvatore", National Research Council (IEOS-CNR), Italy
1/7/2019-10/10/2022: Researcher Institute of Biochemistry and Cell Biology, Italian National Research Council (IBBC-CNR), Italy
2014-2019: Post-Doctoral Researcher Institute of Protein Biochemistry, Italian National Research Council (IBP-CNR), Italy.
2012-2014: Post-Doctoral Researcher Institute of Biostructure and Bioimaging, Italian National Research Council (IBB-CNR), Italy.
2010-2012: Post-Doctoral Researcher Department of Biochemistry and Medical Biotechnology of University of Naples “Federico II”, Italy.
2003-2010: Post-Doctoral Researcher CEINGE Biotecnologie Avanzate s.c.a.r.l., Italy.§
1999–2002: PhD student, Anton Dohrn Zoological Station, Italy.
1998: Research Fellow, Institute of Protein Biochemistry and Enzymology, Italian National Research Council (IBPE-CNR), Italy.
1994-1997: Bachelor of Science thesis fellow, Institute of Protein Biochemistry and Enzymology, Italian National Research Council (IBPE-CNR), Italy.


2014 - 2019: Postdoctoral Research Fellowship at the Institute of Protein Biochemistry - CNR, Naples, Italy.
2012 - 2014: Postdoctoral Research Fellowship at the Institute of Biostructure and Bioimaging – CNR, Naples, Italy.
2010 - 2012: Postdoctoral Research Fellowship at Department of Biochemistry and Medical Biotechnology of University of Naples “Federico II”, Naples, Italy.
2004 - 2007: Fellowship at CEINGE within the agreement between CEINGE and Ministero dell’Istruzione dell’Università e della Ricerca (MIUR), as an expert researcher.
2004: fellowship funded by FIRC (Italian Fondation for Cancer Research)


Golgi Complex: A Signaling Hub in Cancer.
Cells. 2022 Jun 21;11(13):1990.
Spano D, Colanzi A.

Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2.
Nat Commun. 2016 May 31;7:11727
Maria Luisa Barretta, Daniela Spano, Chiara D’Ambrosio, Romina Ines Cervigni, Andrea Scaloni, Daniela Corda and Antonino Colanzi

JNK2 controls fragmentation of the Golgi complex and the G2/M transition through phosphorylation of GRASP65.
J Cell Sci. 2015 Jun 15;128(12):2249-60.
Cervigni RI, Bonavita R, Barretta ML, Spano D, Ayala I, Nakamura N, Corda D, Colanzi A.

Dipyridamole prevents triple-negative breast-cancer progression.
Clin Exp Metastasis. 2013 Jan;30(1):47-68.
Spano D, Marshall JC, Marino N, De Martino D, Romano A, Scoppettuolo MN, Bello AM, Di Dato V, Navas L, De Vita G, Medaglia C, Steeg PS, Zollo M.

Galectin-1 and its involvement in hepatocellular carcinoma aggressiveness.
Mol Med. 2010 Mar;16(3-4):102-15.
Spano D, Russo R, Di Maso V, Rosso N, Terracciano LM, Roncalli M, Tornillo L, Capasso M, Tiribelli C, Iolascon A.


Where we are

Via Pietro Castellino 111
80131 Naples
Tel. 0039 081 6132 579

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