Biophotonics And Advanced Microscopies
Keywords: Raman microscopy, SERS, correlative imaging, bionsensors, biophotonics
Group leader: Anna Chiara De Luca
Contacts:
Phone: +39 0816132612
Lab: +39 08161325206
Email: annachiara.deluca@cnr.it
a.deluca@ieos.cnr.it
Our group is interested in the development of optical technologies based on Raman spectroscopy in order to provide fast and reliable procedures for the biochemical label-free characterization of single cells and molecules.
On a cellular scale, our group aim to provide specific information through optical fingerprinting of cells and tissues and the application of that information for the accurate detection and grading of cancers in biopsy specimens. There are three components to our approach:
- Enhancement of Raman spectroscopy using advanced optics to remove the fluorescence background, increase the resolution and reduce acquisition time and noise;
- Application of this methodology to clinical cell samples (tissue and liquid biopsy), with the ultimate aim of generating a laboratory device (in particular a compact Raman flow cytometer) that will improve cancer diagnosis
- Integration/correlation of Raman microscopy with other microscopies (including surface-enhanced Raman microscopy, structured illumination, mass imaging, fluorescence microscopy, digital holography, electron microscopy) to univocally identify the label-free Raman signatures of molecules of interest and track them inside the samples.
On a molecular scale, we aim to detect low concentration of specific molecules (biosensing) that could be used as reliable biomarkers for cancer diagnosis and prognosis. To realize this goal, we are interested in developing and characterizing custom-made metallic nano-structures (SERS substrates) for monitoring of drug release or for applications in biotechnology and biomedicine.
SERS assisted sandwich immunoassay platforms for ultrasensitive and selective detection of human Thyroglobulin
Biosens & Bioelectron 233, 115322 (2023)
S Spaziani, G Quero, S Managò, G Zito, D Terracciano, PE Macchia, F Galeotti, M Pisco, AC De Luca*, A Cusano
SERS Quantification of Galunisertib Delivery in Colorectal Cancer Cells by Plasmonic-Assisted Diatomite Nanoparticles
Small 17 (34), 2101711 (2021)
S. Managò, C. Tramontano, D. Delle Cave, G. Chianese, G. Zito, L. De Stefano, M. Terracciano, Enza Lonardo, A.C. De Luca*, I. Rea
Bioderived three-dimensional hierarchical nanostructures as efficient surface-enhanced Raman scattering substrates for cell membrane probing
ACS Appl. Mater. Interf. 10(15) 12406 (2018)
S. Managò, G. Zito, A. Rogato, M. Casalino, E. Esposito, A.C. De Luca*, E. De Tommasi
A reliable Raman-spectroscopy-based approach for diagnosis, classification and follow-up of B-cell acute lymphoblastic leukemia
Sci. Rep. 6 24821 (2016)
S. Managò, C. Valente, P. Mirabelli, D. Circolo, F. Basile, D. Corda, A. C. De Luca
Reproducible Surface-Enhanced Raman Quantification of Biomarkers in Multicomponent Mixtures
ACS Nano 8, 2575 (2014)
A.C. De Luca, P. Reader-Harris, M. Mazilu, S. Mariggiò, D. Corda, A. Di Falco
Online fluorescence suppression in modulated Raman spectroscopy
Anal. Chem. 82, 738 (2010)
A.C. De Luca, M. Mazilu, A. Riches, S. Herrington, K. Dholakia
Researcher:
Maria Mangini; PhD in Cell Biology
email:m.mangini@ieos.cnr.it; maria.mangini@ieos.cnr.it
Post-doctoral fellows:
Deniz Yilmaz; PhD in Biotechnology
email: d.yilmaz@ieos.cnr.it; deniz.yilmaz@ieos.cnr.it
Alessandro Esposito; PhD in Nanotechnology
email: al.esposito@ieos.cnr.it; alessandro.esposito@ieos.cnr.it
Hossein Kadhem; PhD In Physics
email: h.kadhem@ieos.cnr.it
Miriam Lucariello; PhD in Cell Biology
email: m.lucariello@ieos.cnr.it
Post-graduate fellow:
Somayeh Farazpuor
email: somayeh.farazpour@gmail.com
Graziano Preziosi
email: grazianopreziosi7@gmail.com
2022 – 2026 PNRR Infrastructure grant - SEE LIFE IR00023
2022 – 2024 SeeInvis @CNR grant
2019 – 2024 AIRC IG Grant n.21420
Project title: Correlative optical microscopies for cancer imaging
2019 – 2023 Progetto Ricerca Finalizzata (under 40) GR-2018-12367517
Project title: Pharmacological targeting of CtBP1/BARS in cancer
2022 – 2024 Bilateral agreement CNR/ASRT
Project title: Synthesis and biological evaluation of novel Triazole based compounds as
anti-cancers and non-steroidal Aromatase Inhibitors
2018 – 2022 PON Ricerca e Innovazione 2014-2020
Project title: Nanofotonica per nuovi approcci diagnostici e terapEutici in Oncologia e
Neurologia (NeON)- ARS01_00769
2018 – 2022 POR CAMPANIA FESR 2014/2020
PROGETTO CIRO - Campania Imaging Infrastructure for Research in Oncology
2018 – 2022 POR CAMPANIA FESR 2014/2020
PROGETTO PLATT - Piattaforma innovativa per lo sviluppo, di nuovi radiofarmaci per
diagnosi e cura di neoplasie solide e nuovi potenti metodi non-invasivi per la diagnosi e
gestione del cancro ai polmoni
2018 – 2022 PON Potenziamento Infrastruttura Europea
Progetto IMPARA - Imaging dalle molecole alla preclinica- PIR01_00023
2012 – 2018 AIRC start up grant n. 11454
Project Title: Advanced photonic techniques for efficient non-invasive cancer diagnosis
at cellular and molecular scale.
2012 – 2018 Finanziamento Premiale FOE 2014-Italian Ministry for Research (MIUR)
Project Title: Tecnologie Innovative per la Terapia con Radionuclidi e l'Imaging
Biologico (TERABIO)
2013 – 2017 FIRB from Italian Ministry for Research (MIUR) n. RBFR12WAPYProject title: Photonic techniques for early cancer diagnosis at cellular and molecular scale
Tumore alla tiroide, in Campania uno studio che scopre le metastasi tramite un nanosensore
È pronto il nanosensore che riesce a individuare le metastasi del tumore alla tiroide: sfruttando la luce laser e minuscole sfere d'oro che ne potenziano la sensibilità, riesce a trovarle in modo affidabile rilevando la presenza di un'unica proteina. Lo hanno messo a punto i ricercatori di tre istituti del Consiglio Nazionale delle Ricerche di Napoli e delle Università del Sannio e Federico II di Napoli. Lo studio, pubblicato sulla rivista Biosensors and Bioelectronics, utilizza una tecnica che ha un enorme potenziale nella realizzazione di sensori biologici e che potrebbe essere estesa anche ad altre forme di tumore, facilitando la diagnosi e l'individuazione dei trattamenti più efficaci.
Congratulations to Maria Mangini of the Biophotonics and Advanced Microscopy Lab for the best poster award from SIB- Società Italiana di Biochimica e Biologia Molecolare.
Congratulations!!! We look forward to further excellent scientific achievements!
Biomedical Optics Express Top Downloads: May and September 2022
The Invited Review Article “Diatom biosilica in plasmonics: applications in sensing, diagnostics and therapeutics” by Edoardo De Tommasi (CNR-ISASI) and Anna Chiara De Luca (CNR-IEOS) has been among the top 10 downloads of Biomedical Optics Express in May 2022!
Access to the paper
https://opg.optica.org/boe/fulltext.cfm?uri=boe-13-5-3080&id=472316
“SERS Quantification of Galunisertib Delivery in Colorectal Cancer Cells by Plasmonic-Assisted Diatomite Nanoparticles” has been published on the Small cover!
Access to the paper
https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202170178
Press releases:
https://ladiscussione.com/113037/salute/un-nanosistema-per-contrastare-il-tumore-al-colon/
https://tg24.sky.it/salute-e-benessere/2021/07/30/tumore-colon-cnr
https://www.cnr.it/it/comunicato-stampa/10498/un-nanosistema-per-contrastare-il-tumore-al-colon
https://www.airc.it/traguardi-dei-ricercatori/un-nuovo-nanosistema-per-il-dosaggio-specifico-di-farmaci-antitumorali-0921
Internalization kinetics and cytoplasmic localization of functionalized diatomite nanoparticles in cancer cells by Raman imaging
Access to the paper
https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201700207
